Invitrogen™ MAXIscript™ T7 Transcription Kit
Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations
Brand: Invitrogen™ AM1312
Additional Details : Weight : 0.02000kg
The Ambion™ MAXIscript™ T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 30 reactions. The MAXIscript In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/μg in just 10 minutes. MAXIscript Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb.
- High yields of high specific activity, full-length RNA probes in just 10 minutes
- Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization
- Includes TURBO DNase™
- All kits contain quality-tested DNase/RNase-free reagents
- RNA Yield: 2 to 6μg
|In Vitro Transcribed RNA, Probe|
|In Vitro Transcription|
|T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.|
|PCR Products, Linearized DNA|
For Research Use Only. Not for use in diagnostic procedures.