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Invitrogen™ FirstChoice™ RLM-RACE sæt

Artikelnummer. 10554445
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Mængde:
1 sæt
Pakningsstørrelse:
Hver
Inkluderer:
Kit with manual, FastAP™ Thermosensitive Alkaline Phosphatase, 10X FastAP Buffer, Tobacco Acid Pyrophosphatase, 10X TAP Buffer, T4 RNA Ligase, 10X T4 RNA Ligase Buffer, M-MLV Reverse Transcriptase, RNase Inhibitor, 10X RT Buffer, 2.5 mM dNTP Mix, Mouse Thymus RNA (1 mg/ml), Random Decamers, 5’ RACE Adapter, 3’ RACE Adapter, 5’ RACE Outer Primer, 5’ RACE Inner Primer, 3’ RACE Outer Primer, 3’ RACE Inner Primer, 5’ RACE Outer Control Primer, 5’ RACE Inner Control Primer, 5’ PCR Control Primer, 3’ RACE Control Primer and Ammonium Acetate Stop Solution, Nuclease-free Water
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Artikelnummer. Mængde unitSize Inkluderer
10554445 1 sæt Hver -
10066184 - Hver Kit with manual, FastAP™ Thermosensitive Alkaline Phosphatase, 10X FastAP Buffer, Tobacco Acid Pyrophosphatase, 10X TAP Buffer, T4 RNA Ligase, 10X T4 RNA Ligase Buffer, M-MLV Reverse Transcriptase, RNase Inhibitor, 10X RT Buffer, 2.5 mM dNTP Mix, Mouse Thymus RNA (1 mg/ml), Random Decamers, 5’ RACE Adapter, 3’ RACE Adapter, 5’ RACE Outer Primer, 5’ RACE Inner Primer, 3’ RACE Outer Primer, 3’ RACE Inner Primer, 5’ RACE Outer Control Primer, 5’ RACE Inner Control Primer, 5’ PCR Control Primer, 3’ RACE Control Primer and Ammonium Acetate Stop Solution, Nuclease-free Water
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Artikelnummer. 10554445 Leverandør Invitrogen™ Leverandørnr. AM1700

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7888.00 DKK valid until 2026-03-27
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Beskrivelse

Indeholder

Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5M EDTA should be stored at –20°C. Nuclease-free water may be stored at any temperature.

Kræver

SuperTaq TermostabilTaq DNA-polymerase sælges separat. For optimal amplifikation af fragmenter≥ 1 kb, brug SuperTaq-Plus.

Designet til kun at amplificere cDNA fra fuldlængde, capped mRNA, som normalt producerer et enkelt bånd efter PCR

FastAP™ Thermosensitive Alkaline Phosphatase, 10X FastAP Buffer, Tobacco Acid Pyrophosphatase, 10X TAP Buffer, T4 RNA Ligase, 10X T4 RNA Ligase Buffer, M-MLV Reverse Transcriptase, RNase Inhibitor, 10X RT Buffer, 2.5 mM dNTP Mix, Mouse Thymus RNA (1 mg/ml), Random Decamers, 5' RACE Adapter, 3' RACE Adapter, 5' RACE Outer Primer, 5' RACE Inner Primer, 3' RACE Outer Primer, 3' RACE Inner Primer, 5' RACE Outer Control Primer, 5' RACE Inner Control Primer, 5' PCR Control Primer, 3' RACE Control Primer and Ammonium Acetate Stop Solution should be stored at -20°C. Nuclease-free Water may be stored at any temperature.

De FirstChoice RLM-RACE Kit er en væsentlig forbedring i forhold til den grundlæggende hurtige amplifikation af cDNA-ender (RACE) protokol. RLM-RACE-proceduren udvælger kun fuldlængde-mRNA —uden rRNA, tRNA eller nedbrudt —RNA og letter kloningen af sekvenser fra 5'-enderne af meddelelser.

  • Fra RNA til PCR-produkt på mindre end et døgn
  • Giver enkelt, specifikt produkt fra sjældne transkriptioner
  • Vælger 5' og/eller 3' ender af sande beskeder
  • Effektiv—
  • Al enzymatiske reaktioner er optimeret for at sikre påvisning af selv sjældneste mRNA
  • Slutproduktstørrelse: 7 kb eller mindre
  • Optimal reaktionstemperatur: 42 °C
  • Prøve: RNA

  • Hurtig amplifikation af cDNA-ender (5'-RACE) er polymerasekædereaktionsbaseret teknik udviklet til at lette kloning af sekvens fra 5'-enderne af beskeder
  • Forbedring af grundlæggende RACE-protokol
  • Vælger fuld-længde mRNA —uden rRNA, tRNA eller nedbrudt mRNA

Optimal reaktionstemperatur: 42 °C

Kloning, PCR og realtids-PCR, omvendt transkription, cDNA-biblioteker og bibliotekskonstruktion

Ordreinfo

Forsendelsestilstand: Tøris

TRUSTED_SUSTAINABILITY

Tekniske data

Produkttype RLM-RACE sæt
Inkluderer Kit, FastAP™ Thermosensitive Alkaline Phosphatase, 10X FastAP Buffer, Tobacco Acid Pyrophosphatase, 10X TAP Buffer, T4 RNA Ligase, 10X T4 RNA Ligase Buffer, M-MLV Reverse Transcriptase, RNase Inhibitor, 10X RT Buffer, 2.5 mM dNTP Mix, Mouse Thymus RNA (1 mg/ml), Random Decamers, 5’ RACE Adapter, 3’ RACE Adapter, 5’ RACE Outer Primer, 5’ RACE Inner Primer, 3’ RACE Outer Primer, 3’ RACE Inner Primer, 5’ RACE Outer Control Primer, 5’ RACE Inner Control Primer, 5’ PCR Control Primer, 3’ RACE Control Primer and Ammonium Acetate Stop Solution, Nuclease-free Water
Indhold og opbevaring

•FastAP™ Thermosensitive Alkaline Phosphatase
•10X FastAP Buffer, Tobacco Acid Pyrophosphatase
•10X TAP Buffer, T4 RNA Ligase
•10X T4 RNA Ligase Buffer
•M-MLV Reverse Transcriptase
•RNase Inhibitor
•10X RT Buffer
•2.5 mM dNTP Mix
•Mouse Thymus RNA (1 mg/ml)
•Random Decamers
•5' RACE Adapter
•3' RACE Adapter
•5' RACE Outer Primer
•5' RACE Inner Primer
•3' RACE Outer Primer
•3' RACE Inner Primer
•5' RACE Outer Control Primer
•5' RACE Inner Control Primer
•5' PCR Control Primer
•3' RACE Control Primer
•Ammonium Acetate Stop Solution
•Components should be stored at -20°C. Nuclease-free Water may be stored at any temperature.
Endelig produkttype cDNA
Optimal reaktionstemperatur 42 °C
Omvendt transkriptase M-MLV
Forsendelsestilstand Tøris
Format Sæt
Produktlinje Ambion , FirstChoice
Til brug med (applikation) cDNA-biblioteker og bibliotekskonstruktion
Mængde 1 sæt
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