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Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

6780.00 DKK - 29060.00 DKK

Specifications

Detection Method Primer-probe
Reaction Speed Fast or Standard
For Use With (Equipment) 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Product Line CellsDirect™, Platinum™, SuperScript™
For Use With (Application) RT-PCR
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Includes: 10mL Resuspension Buffer; 1mL Lysis Enhancer; 500μL DNase I, Amplification Grade (1U/μL); 160μL 10X DNase I Buffer; 400μL 25mM EDTA; 100μL SuperScript III RT/Platinum Taq Mix; 2 x 1.25mL 2X Reaction Mix; 1mL 50mM MgSO4; 2mL DEPC-treated water; 10μL HeLa Total RNA (10ng/μL); 100μL ROX Reference Dye

Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10043982
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Invitrogen™
11753100
100 reactions
6780.00 DKK
100 reactions
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10308782
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Invitrogen™
11753500
500 reactions
29060.00 DKK
500 reactions
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Estimated Shipment: 19-04-2024
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Description

Description

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Efficiency: offers significant time and cost savings for increased throughput
Convenience: enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
Sensitivity: no separate RNA isolation step increases sensitivity by minimizing sample loss
Broad detection range: detect targets from samples ranging from a single cell to 10,000 cells
Versatility: compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)

Streamline your workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for your qRT-PCR application
CellsDirect technology is validated for gene expression studies involving limited samples. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™ or LUX™ fluorogenic primers).

This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit with ROX Reference Dye included in the 2X Reaction Mix is also available.

Order Info

Shipping Condition: Dry ice

Specifications

Specifications

Primer-probe
7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
RT-PCR
Cells
Fast or Standard
CellsDirect™, Platinum™, SuperScript™
2X
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.