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Abnova ES-D3 (Mouse pluripotent embryonic stem cell) whole cell lysate, nondenatured

Artikelnummer. 16019615
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Mængde:
50 μg
Pakningsstørrelse:
50µg
Artikelnummer. Mængde unitSize
16019615 50 μg 50µg
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Artikelnummer. 16019615

Brand: Abnova L044V2.50ug

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Descrizione

  • Stem cells maintained in the undifferentiated state using specialized culturing protocols
  • Monitored for undifferentiated cell morphology
  • Analyzed using Flow Cytometry against specific cell marker
  • Coommasie Blue Staining of total cell lysate in SDS-PAGE

Western Blotting, Immunoprecipitation

Specifica

Til brug med (applikation) Immunoprecipitation, Western Blot
Væv Embryonic
Oversigt Whole cell lysate (non-denatured)
Lyseringsbuffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Fremstillingsmetode Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Kvalitetskontrol test 12.5% SDS-PAGE Stained with Coomassie Blue.
Mængde 50 μg
Opbevaringsbuffer In modified RIPA Lysis Buffer.
Værtsarter Mouse
Koncentration 4 mg/mL
Indhold og opbevaring Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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