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A-431 (human epidermoid carcinoma) Nuclear extract lysate, Non-denatured; Abnova

Artikelnummer. 16029545
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Mængde:
50 μg
Pakningsstørrelse:
50µg
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Artikelnummer. 16029545

Brand: Abnova L015V4.50ug

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Beskrivelse

Non-denatured nuclear extract lysate of A-431 (human epidermoid carcinoma)

  • Tissue: Epidermis
  • Host: Human
  • Lysis Buffer:
    • Buffer A: 10mM HEPES pH 7.9, 1.5mM MgCl2, 10mM KCl, 0.5mM DTT.
    • Buffer C: 20mM HEPES pH 7.9, 25%(v/v) Glycerol , 0.42M NaCl , 1.5mM MgCl2, 0.2mM EDTA, 0.5mM DTT & 0.5mM PMSF
    • Buffer D : 20mM HEPES pH 7.9, 20%(v/v) glycerol, 50mM KCl, 0.2mM EDTA, 0.5mM DTT & 0.5mM PMSF
  • Storage buffer: In buffer D

Applications:

Western Blotting, Immunoprecipitation

Tekniske data

Til brug med (applikation) Immunoprecipitation, Western Blot
Væv Epidermis
Oversigt Nuclear extract cell lysate (non-denatured)
Lyseringsbuffer Buffer A: 10mM HEPES pH 7.9, 1.5mM MgCl2, 10mM KCl, 0.5 mM DTT. Buffer C: 20mM HEPES pH 7.9, 25%(v/v) Glycerol , 0.42M NaCl , 1.5mM MgCl2, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF. Buffer D : 20mM HEPES pH 7.9, 20%(v/v) glycerol, 50mM KCl, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF.
Fremstillingsmetode Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C, and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged, divided into aliquots, and stored at -80°C. The protein concentration was determined by the method of Bradford (Bio-Rad protein assay, microplate standard assay). The lysate was adjusted to 2 mg/ml.
Kvalitetskontrol test 12.5% SDS-PAGE Stained with Coomassie Blue.
Mængde 50 μg
Opbevaringsbuffer In Buffer D.
Værtsarter Human
Indhold og opbevaring Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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