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Invitrogen™ 6x-His Tag Monoclonal Antibody (HIS.H8), HRP
Mouse Monoclonal Antibody
Brand: Invitrogen™ MA121315HRP
1439.75 DKK valid until 2025-12-19
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Beskrivelse
6x-His Tag Monoclonal Antibody for Western Blot
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Tekniske data
| 6x-His Tag | |
| Monoclonal | |
| 1 mg/mL | |
| PBS with proprietary stabilizer and 0.07% Kathon; pH 7.2 | |
| Mouse | |
| Protein A | |
| RUO | |
| Tag | |
| Antibody | |
| IgG2b |
| Western Blot | |
| HIS.H8 | |
| HRP | |
| 6His tag; 6X His; His Tag; Histidine Tag | |
| 6x His synthetic peptide. | |
| 50 μL | |
| Primary | |
| 4°C | |
| Liquid | |
| Tag |
Sikkerhed og håndtering
Produktidentifikator
Signalord
Farekategori
Faresætning
Sikkerhedssætning
Supplerende oplysninger
- 6x-His Tag Monoclonal Antibody (HIS.H8), HRP
Signalord
- Advarsel
Farekategori
- Alvorlige øjenskader/øjenirritation Kategori 2
- Hudætsning/hudirritation Kategori 2
- Hudsensibilisering Kategori 1
Faresætning
- H315-Forårsager hudirritation.
- H317-Kan forårsage allergisk hudreaktion.
- H319-Forårsager alvorlig øjenirritation.
Sikkerhedssætning
- P264-Vask grundigt efter brug.
- P280-Bær beskyttelseshandsker/beskyttelsestøj/øjenbeskyttelse/ ansigtsbeskyttelse.
- P302+P352-VED KONTAKT MED HUDEN: Vask med rigeligt vand/.
- P305+P351+P338-VED KONTAKT MED ØJNENE: Skyl forsigtigt med vand i flere minutter. Fjern eventuelle kontaktlinser, hvis dette kan gøres let. Fortsæt skylning.
- P332+P313-Ved hudirritation: Søg lægehjælp.
- P337+P313-Ved vedvarende øjenirritation: Søg lægehjælp.
- P501b-Indholdet/beholderen bortskaffes i henhold til de lokale/regionale/nationale/internationale forskrifter.
Supplerende oplysninger
- MIXTURE LIST-Indeholder: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no. 247-500-7] and 2-methyl-2H-isothiazol-3-one
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Produkttitel
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