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3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity, Thermo Scientific Chemicals Product Code.: 15440187
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3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity, Thermo Scientific Chemicals

Product Code. 15440187
1000 mL
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Regulatory: This product fall under regulation “Lov om kemiske stoffer og produkter - lovbekendtgørelse nr. 1755 af 22. december 2006. If applicable, customers must provide a license before delivery.

Product Code. 15440187

Brand: Thermo Scientific Alfa Aesar J61325.AU

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4625.82 DKK valid until 2025-12-31
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This item is not returnable. View return policy
Regulatory: This product fall under regulation “Lov om kemiske stoffer og produkter - lovbekendtgørelse nr. 1755 af 22. december 2006. If applicable, customers must provide a license before delivery.

Description

3,3’,5,5’-Tetramethylbenzidine (TMB, CAS # 54827-17-7) is a blue chromogenic substance used in staining techniques such as immunohistochemistry.

TMB is a substrate for horseradish peroxidase. Recommended for ELISA (microwell) procedures,3,3?,5,5?-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3?,5,5?-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• TMB is an aromatic amine that acts as a hydrogen donor in horseradish peroxidase-catalyzed reactions
• The transfer of hydrogen results in a blue stain associated with the target of interest
• This pH-dependent color change can be confirmed using spectrophotometric methods
• TMB is commonly used in immunohistochemistry; TMB is light-sensitive, and all applications should be performed in the dark

Application

• TMB is suitable for immunohistochemistry and immunocytochemistry applications including ELISA and cell and tissue staining

TRUSTED_SUSTAINABILITY

Identificadores de productos químicos

CAS 54827-17-7
Molecular Formula C16H20N2
Molecular Weight (g/mol) 240.35
MDL Number MFCD00007748
InChI Key UAIUNKRWKOVEES-UHFFFAOYSA-N
Synonym 3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble
PubChem CID 41206
IUPAC Name 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
SMILES CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

Especificaciones

Color Yellow
Quantity 1000 mL
UN Number UN2810
Beilstein 2808541
Solubility Information Insoluble in water. Soluble in organic solvents such as DMSO,96 % Ethanol,Acetone,Chloroform,Toluene.
Formula Weight 240.35
Physical Form Liquid
Chemical Name or Material 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity

Certificates

Certificates

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5 results found
Lot Number Certificate Type Date Catalog Number
Lot NumberP26L036 Certificate TypeCertificate of Analysis Date28/03/2025 Catalog NumberJ61325.AP,J61325.AU,J61325.=E
Lot NumberM14L017 Certificate TypeCertificate of Analysis Date17/01/2025 Catalog NumberJ61325.AP,J61325.AU,J61325.=E
Lot NumberM14L019 Certificate TypeCertificate of Analysis Date17/01/2025 Catalog NumberJ61325.AP,J61325.AU,J61325.=E
Lot NumberX09K049 Certificate TypeCertificate of Analysis Date17/10/2024 Catalog NumberJ61325.AP,J61325.AU,J61325.=E
Lot NumberX09K048 Certificate TypeCertificate of Analysis Date17/10/2024 Catalog NumberJ61325.AP,J61325.AU,J61325.=E

RUO – Research Use Only

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