Fisher Bioreagents

High-quality, high-purity, and pre-qualified reagents that meet your life science needs, all from one source.

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Products
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Resources

Featured Products

Fisher Bioreagents Ethanol Solutions

Reliable, ready-to-use pure solutions.

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Separate Protein Gels Faster

See the results of comparison experiments.

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Fisher Bioreagents Buffers

Buffers for all your applications.

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Sodium Dodecyl Sulfate (SDS), Micropellets, Fisher BioReagents

Fisher Bioreagents Sodium Dodecyl Sulfate (SDS), Micropellets

Sodium Dodecyl Sulfate is the most commonly used detergent in protein purification and electrophoresis. Tested for Dnase, Rnase and protease to ensure absence of these hydrolyzing enzymes. Mixes easily with acrylamide and tris glycine buffer to make ready to pour.

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Fisher Bioreagents Ethanol, Absolute (200 Proof), Molecular Biology Grade

Ultrapure molecular biology grade ethanol is used for the purification and precipitation of biomolecules such as nucleic acids and proteins. It is also used in histology to prepare staining and destaining reagents and for dehydrating tissues prior to embedding.

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Fisher Bioreagents Water for Life Science

Water plays a fundamental role in life science applications and workflows depending upon properties such as water purity, grade, sterility, and additives. Fisher Bioreagents' range of water is ideal for many sensitive procedures such as PCR, electrophoresis, DNA sequencing, and buffers for enzymatic analyses.

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Top Categories

Enzymes Restriction Enzymes Modifying Enzymes

Buffers Life Science Buffers Restriction Enzyme Buffers

Molecular Biology Reagents and Kits PCR Reagents RNA Preparation and Purification

Gel Electrophoresis Reagents and Buffers Nucleic Acid Molecular Weight Markers Electrophoresis Gel Casting Reagents Electrophoresis Gel Stains

Fisher Bioreagents: Your Source for Life Science Products

Obtaining optimal results in your life science research requires careful selection of reagents. When your experiments require exact conditions, you can depend on the reliability of Fisher Bioreagents™ products for:

QUALITY: Tight specifications
CONSISTENCY: Lot-to-lot uniformity
SELECTION: Powders, granules, concentrated stock solutions, or ready-to-use liquids
PACKAGING: State-of-the-art containers designed for safety and utility
CONVENIENCE: Prequalified for a variety of applications
ECONOMY: Configurations to meet all budgets
SCALE: From bench to batch sizes

Fisher Bioreagents meet the needs of every budget and scale. Economical powders come in a variety of package sizes, while concentrated stock solutions offer convenient one-step dilution. Realise the greatest possible time savings when you choose a Fisher Bioreagents ready-to-use solution.

Restriction Enzymes

Why Choose Fisher Bioreagents™ OPTIZYME™ Restriction Enzymes

Purity: OPTIZYME restriction enzymes are produced using high-yielding expression vectors, resulting in a higher level of purity and lower processing time than native restriction enzymes.

Consistency: OPTIZYME restriction enzymes are manufactured in class D cleanrooms using recombinant production techniques to yield large lot sizes. The combination of leading production technology with ISO9001 quality and ISO14001 environmental management systems ensures high digestion and ligation efficiency, as well as batch-to-batch reproducibility.

Value Pricing: The use of high-yielding expression vectors and bacterial strains enables Fisher Bioreagents to offer recombinant enzymes with the lowest price/unit charges, allowing our customers to benefit from both substantial savings and improved consistency and quality.

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Resources

Fisher BioReagents Relative Activity of OPTIZYME Guide

Filename: 16475_fisher-scientific-bioreagents-relative-activity-optizyme-guide.pdf
Size: 57 KB
Format: application/pdf

Fisher BioReagents Reaction Buffers for OPTIZYME Guide

Filename: 16475_fisher-scientific-bioreagents-reaction-buffers-optizyme-guide.pdf
Size: 64 KB
Format: application/pdf

Fisher BioReagents Recognition Sequences for OPTIZYME Guide

Filename: 16475_fisher-scientific-bioreagents-recognition-sequences-optizyme-guide (1).pdf
Size: 62 KB
Format: application/pdf

Fisher BioReagents Site-Specific Methylation on OPTIZYME Guide

Filename: 16475_fisher-scientific-bioreagents-site-specific-methylation-optizyme-guide.pdf
Size: 98 KB
Format: application/pdf

Bioreagent Buffers

Buffers for Every Budget and Scale

Economical powders come in various package sizes
Concentrated stock solutions provide convenience with one easy-dilution step
Ready-to-use solutions offer the greatest possible time savings

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Fisher Bioreagents™ CellPURE™ Biological Buffers

CellPURE Biological Buffers from Fisher Bioreagents are ultrapure zwitterionic buffers that possess both positive and negative charges. CellPURE Biological Buffers are ideal for cell cultivation, isolation of cells, enzyme assays, and other biochemical applications. They are manufactured under strict quality control guidelines to ensure performance and reliability.

Advantages

Cell culture tested
Analyzed for the absence of nucleases and proteases
Tested for endotoxin and bioburden levels
pKa values mostly independent of temperature and concentration
High water solubility
Minimal permeability to biological membranes

Applications

Tissue culture media and maintenance of cell lines
Enzyme assays
Electrophoresis of RNA
Transfection of mammalian cells

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Relative Activity of OPTIZYME™ Restriction Enzymes

Reaction Buffers for OPTIZYME™ Restriction Enzymes

Popularly known today as “Good” buffers, these organic buffers have several advantages compared to traditional inorganic buffering systems such as phosphate, borate or bicarbonate.

“Good” buffers approach the “ideal” buffer state by having pKa values at or near physiological pH and minimal interference with biological processes. JustPURE “Good” buffers from Fisher Bioreagents have very high purity (assay >99%) and only trace amounts of metal ions, which makes them useful for applications requiring tight control of elemental content.

Ultrapure zwitterionic buffers
Optimized for research in cellular and molecular biology
Low interference with biological reactions

Advantages

pKa values mostly independent of temperature and concentration
High water solubility
Chemically and enzymatically stable
Minimal permeability to biological membranes
Limited effect on biochemical reactions
Minimal absorption in spectral range 240nm to 700nm

Applications

High efficiency transfection of mammalian cells
Gel electrophoresis of RNA
Protein isolation applications
Cell cultures and enzyme assays
Bioanalytical methods such as IEF, 2-D electrophoresis, and SDS-PAGE

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Technical Tip

CellPURE Biological Buffers are of the highest quality (purity ≥99%). They are tested for low heavy metal content and the absence of endotoxins, nucleases, and proteases. It is important to use only water of the highest quality to prepare the buffer solutions. Water that stands too long in pipes or water produced by a system needing routine maintenance increases the risk of contamination of buffer solutions. Fisher BioReagents provides several grades of high-purity water suitable for the preparation of high-quality buffer solutions.

Resources

Vital Reagents, Essential Products for Life Science Research

Filename: 16475_Vital_Reagents.pdf
Size: 2 MB
Format: application/pdf

Buffer Guide

Filename: Fisher_Bioreagents_Buffer_Guide.pdf
Size: 14 MB
Format: application/pdf

Antibiotics and Antimycotics

Optimized for Cell Culture

In many life science laboratories, the in vitro culturing of bacterial, plant, and animal cells is a routine task. High-quality antibiotics and antimycotics from Fisher BioReagents can be used to ensure successful growth of cells by eliminating unwanted bacterial strains and fungi, while maintaining the health and vitality of the desired cells.

Advantages

High quality - antibiotics and antimycotics meet rigorous quality control tests, including verification of potency, purity, and solubility using microbiological and chromatographic methods
Built for selection - excellent choice of antibiotics for selection of antibiotic resistance genes in both bacterial and mammalian cells
High purity and stability - ultrapure antibiotics ensure successful growth of target cells and reproducible results
Maximum convenience - antibiotics and antimycotics come in both sterile powder and liquid forms

Applications

Plant cell culture
Mammalian cell culture
Tissue culture
Genetic marker selection

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Water

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Workflow

Molecular Biology

We offer a range of ultra pure, high-quality reagents for a variety of molecular biology applications such as nucleic acid electrophoresis, nucleic acid purification and sequencing, PCR, and more.

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Protein Chemistry

From immunodetection reagents to protein purification, we offer a range of high-quality reagents that are pre-qualified for your protein research needs.

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Cell Biology

We offer a range of high-purity reagents that have been optimized for various cell and tissue culture applications including antibiotics, amino acids, and media.

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Electrophoresis

Fisher Bioreagents offers a wide range of reagents for electrophoresis applications. Choosing the right reagents will allow you to achieve optimal results in your experiment. Use our quick selection guides to assist you in finding the right grades of agarose, buffers and ladders.

Compared to polyacrylamide gel, agarose has a lower resolution but wider range of separation. Lower grades of agarose can be contaminated with other polysaccharides, salts and proteins. Such impurities can alter the gelling/melting temperature of agarose solutions or affect the ability to use the recovered nucleic acid sample in a post-electrophoresis application.

Fisher BioReagents offers three different grades of agarose that are functionally tested and pre-qualified for specific applications. Agarose grades for use in electrophoresis of nucleic acids include:

Genetic Analysis Grade - Agarose that yields biologically active DNA or RNA. Testing includes enzymatic performance measurements
Molecular Biology Grade - Suitable for analytical separation of DNA or RNA
PCR Grade - The original agarose for analytical separation of PCR amplicons (<1kb)

Type of Agarose	Low EEO	Low Melting	Wide Separation Range	PCR Grade
Recovery of DNA and RNA	X	X	X	X
Southern and Northern Biots	X
DNA/RNA Separation 50bp to 1kb				X
DNA/RNA Separation >1kb	X	X	X
PCR Fragment Analysis	X	X	X	X
In-Gel Reactions (Ligation, Transformations, PCR)
Colony Lifts	X
Available Pack Sizes	100g to 500g	25g	100g to 500g	100g
Agarose Grade	Molecular Biology	Molecular Biology	Genetic Analysis	PCR

Two buffers commonly used for DNA agarose electrophoresis are Tris-acetate with EDTA (TAE; 40mM Tris-acetate, 1mM EDTA) and Tris-borate with EDTA (TBE, 89mM Tris-borate, 2mM EDTA). TAE and TBE have different properties, which makes one more suitable than the other for a specific purpose.

Fisher BioReagents provides a wide range of standards, including routine DNA ladders for quick size and quality assessment as well as Fisher Bioreagents™ exACTGene™ DNA ladders that allow for quantitative analysis.

Fisher Bioreagents™ RiboLadders™ RNA Standards

These standards can be used to assess single-stranded RNA molecules on both native and denaturing agarose gels. These unique RNA standards are lyophilized to reduce thawing-related degradation, to prolong shelf life, and to ensure consistent performance.

exACTGene and Routine DNA Ladders

Ready-to-use (pre-mixed with the loading dye), room temperature, stable DNA ladders are available for all common electrophoresis applications.

Application	Low EEO	Numbers of Bands	Number of Loadings
exACTGene DNA ladders are ideal for qualitative analysis, quantitative estimation, and size assessment
PCR fragment analysis	25-650bp	14	100/10μL
PCR fragment analysis, small DNA digests	25-1,000bp	12	100/10μL
Quick check of PCR or enzyme digestion results	50-2,000bp	8	100/10μL
General purpose, small DNA fragments	100-1,000bp	10	100/10μL
Fast run times, small DNA fragments	100-2,000bp	8	100/10μL
Clone identification	100-2,686bp	14	100/10μL
Large size PCR or cloning	100-2,000bp	10	100/10μL
Small or large cloning application	100-5,000bp	16	100/10μL
General purpose, large digested DNA	300-10,000bp	13	100/10μL
General purpose, wide size range	100/10,000bp	19	100/10μL
General purpose, extra-large fragments	300-24,000bp	15	100/10μL
Routine DNA ladders are designed for qualitative analysis and size assessment
Small fragments, quick size assessment	50-2,000bp	11	200/5μL
Quick size assessment of broad size range	50-10,000bp	16	200/5μL

Fisher Bioreagents

Vital Reagents for Life Science Research

A variety of grades are available from Fisher BioReagents to support your specific application.

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Fisher bioreagents buffer guide

Bringing Chemistry to Life.

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Water and Core Products for Molecular Biology

Fisher BioReagents Water, Molecular Biology Grade is ideal for many fundamental procedures such as PCR, electrophoresis, DNA sequencing, and buffers for enzymatic analyses.

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Ethanol Grades

The Thermo Scientific™ and Fisher Bioreagents™ range of Molecular Biology Grade Ethanol now includes 70%, 80%, 96%, and 100% ethanol solutions.

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Fisher Bioreagents

Lysis Buffers

Within our Thermo Fisher Scientific Laboratory Chemicals portfolio we offer all the necessary products to prepare lysis buffer solutions with the correct specifications and in a range of convenient pack sizes. We also offer specialized custom services and can provide the products in bulk quantities if required.

Filename: Lysis_Buffers.pdf
Size: 5 MB
Format: application/pdf

Fisher BioReagents Cloning Troubleshooting Guide

This troubleshooting guide for cloning includes a listing for problems, causes, and solutions.

Filename: fisher-scientific-bioreagents-troubleshooting-cloning-guide.pdf
Size: 67 KB
Format: application/pdf

Fisher BioReagents Cleavage Efficiency Guide

This guide lists the activities of OPTIZYME restriction enzymes when their target sites are located close to the ends of PCR products.

Filename: fisher-scientific-bioreagents-cleavage-efficiency-pcr-fragments-guide.pdf
Size: 52 KB
Format: application/pdf

Fisher BioReagents Star Activity of Restriction Enzymes Guide

This guide lists the enzymes that may exhibit star activity, especially under reaction conditions that deviate from those recommended.

Filename: fisher-scientific-bioreagents-star-activity-enzymes-guide (1).pdf
Size: 68 KB
Format: application/pdf

Fisher BioReagents Troubleshooting Unexpected Cleavage Pattern Guide

Troubleshooting Guide for DNA Digestion: Unexpected Cleavage Pattern and Gel Shift. Table lists possible causes and recommended solutions.

Filename: fisher-scientific-bioreagents-troubleshooting-cleavage-pattern-guide.pdf
Size: 54 KB
Format: application/pdf

Focus on Electrophoresis

Fisherbrand, Fisher Chemical and Fisher BioReagents working together to deliver reliable and essential products that meet your most demanding electrophoresis applications.

Filename: 13094_FOCUS_ph_electrophoresis (1).pdf
Size: 114 MB
Format: application/pdf

Fisher BioReagents Digestion of Agarose Embedded DNA Guide

This guide for digestion of agarose embedded DNA includes a listing of units of enzymes needed for DNA cleavage in 16hours.

Filename: fisher-scientific-bioreagents-digestion-agarose-dna-guide (1).pdf
Size: 46 KB
Format: application/pdf

Fisher BioReagents Troubleshooting Guide for DNA Digestion Guide

Incomplete digestion or no digestion. Table includes a listing of possible causes and recommended solutions.

Filename: fisher-scientific-bioreagents-troubleshooting-dna-digestion-guide.pdf
Size: 80 KB
Format: application/pdf

Fisher BioReagents Molecular Diagnostics Guide

Molecular diagnostics is the fastest-growing field of laboratory science. Fisher BioReagents products are ultrapure and tested for molecular applications.

Filename: fisher-scientific-bioreagents-molecular-diagnostics-guide (1).pdf
Size: 724 KB
Format: application/pdf

Fisher BioReagents SurePrep Purification Kits Guide

SurePrep Purification Kits for isolating RNA, DNA, and proteins in clinical, forensic, and medical research.

Filename: fisher-scientific-bioreagents-sureprep-purifications-kits-guide.pdf
Size: 1002 KB
Format: application/pdf

Fisher Bioreagents

Featured Products

Fisher Bioreagents Ethanol Solutions

Fisher Bioreagents Buffers

Top Categories

Fisher Bioreagents: Your Source for Life Science Products

Resources

Agarose

Buffer

Ladders

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