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IBA Lifesciences

IBA Lifesciences provides high performance research tools for cell and protein isolation based on their proprietary Strep-tag™ technology. Their product portfolio offers solutions for the entire production chain of recombinant proteins, from cloning, transfection, protein expression and purification to detection, immobilization and assays, as well as innovative cell isolation and expansion tools.

 

IBA's Cell Selection and Expansion

TACS - Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection:

  • High purity, yield and viability
  • Label-free, non-activated cells due to fully reversible binding
  • From whole blood, buffy coat, PBMCs or murine samples
  • Affinity chromatographic, magnetic or fluorescent cell separation
TACS - Traceless Affinity Cell Selection

MHC I Streptamer™

Detection and isolation of antigen-specific T-cells with MHC I Streptamers™:

  • Label-free cell isolation according to antigen specificity
  • High specificity, sensitivity, and reproducibility
  • From single cell suspensions (e.g., PBMCs or murine samples)
  • Affinity chromatographic, magnetic or fluorescent cell separation
MHC I Streptamer™

Cell Stimulation and Expansion

CD3: CD28 Streptamers™:

  • Completely reversible reagents
  • Termination of stimulation at any given point in time
  • Adjustable CD3: CD28 ratio 
  • Bead-free

Peptides for T-Cell Stimulation:

  • Purity >85%
  • Endotoxin-free
Cell Stimulation & Expansion

Fluorescent Cell Staining

Detection of antigen-specific T-cells with MHC I Streptamers™ and of receptor-specific T-cells with Fab Streptamers™:

  • Fully reversible
  • High specificity, sensitivity, and reproducibility
  • No leftover staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Fluorescent Cell Staining

Protein Production and Assay

Cloning

More than 150 Twin-Strep-tag™/Strep-tag™II expression vectors:

  • Variety of Twin-Strep-tag™/Strep-tag™ II combined with His, FLAG or GST
  • Different N-/C-terminal orientations
  • Expression hosts: E. coli, mammalia, insect cells, yeast
  • Easy cloning via StarGate vectors
Cloning

Mammalian Expression

The MEXi mammalian expression system is optimised for efficient protein expression and Strep-tag™ purification in mammalian cells:

  • Perfectly matched HEK293E cell line and cell culture media
  • Transient expression for time and cost efficient protein production
  • Multiple expression vectors with different features
Mammalian Expression

Protein Purification

Strep-tag™ technology from the original manufacturer:

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (>95%)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, and protein complexes amongst others
Protein Purification

Detection

For Western blot analysis, immunofluorescence, FACS and ELISA.

Two different detection systems are provided:

  • Strep-Tactin™ and Strep-Tactin™ XT conjugate for Twin-Strep-tag™/Strep-tag™ II proteins
  • Monoclonal antibodies against Twin-Strep-tag™/Strep-tag™ II
Detection

Immobilization

With near covalent binding affinity, – Immobilisation by Strep-Tactin XT: Twin-Strep-tag can be used for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, surface plasmon resonance (SPR) experiments (Biacore) or bio-layer interferometry (BLI):

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated
Immobilisation

Surface-Marker Specific Exosome Isolation

Quick and simple isolation of unlabeled exosomes using Fab-TACS™ for exosomes, which offers:

  • Highly specific and reproducible isolation
  • Minimal contamination by other EVs
  • Label-free exosomes with intact biological functions
  • Source-independent application
  • Reversible capture and release of exosomes
  • A quick and simple protocol, without magnetic beads or ultracentrifugation
Surface-marker Specific Exosome Isolation

Immobilization with Near Covalent Binding Affinity

Reversible capture and analysis of Twin-Strep-tag™ fusion proteins for bio-layer interferometry (BLI) or surface plasmon resonance (SPR) experiments:

  • Indirect immobilization of the ligand prevents it from being subject to inactivation or influences of the immobilization procedure
  • High specificity and affinity in low pM range of the Twin-Strep-tag™ - Strep-Tactin™ XT interaction
  • Ligands can be captured directly from complex samples
  • Strep-Tactin™ XT biosensors/chips can be easily regenerated by an established procedure, whereby further ligand-analyte interactions can be analyzed with the same biosensor/chip
Immobilization with Near Covalent Binding Affinity

Our Perfect Tool for Isolation of Proteins or Complexes

MagStrep 'Type 3' XT Magnetic Beads offer:

  • High specificity and low background resulting in high protein purity
  • High binding affinity and capacity for Strep-tag™ II or Twin-Strep-tag™ fusion proteins
  • Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) in batch format and/or high throughput screening for protein purification via pull-downs
  • Suitable for usage with (semi-)automated magnetic bead purification systems (e.g., Thermo Scientific™ KingFisher™) for automated workflows
Our Perfect Tool for Isolation of Proteins or Complexes

Efficient Purification Even of Large Proteins

The Strep-Tactin™ XT 4Flow™ Starter Kit consists of everything you need for a quick start:

  • Strep-Tactin™ XT 4Flow™ gravity flow column
  • All buffers required for washing, elution, and regeneration
  • Strep-Tactin™ conjugated with horseradish peroxidase (HRP; for Western blot detection)
  • Short protocol
Efficient Purification Even of Large Proteins

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

Applicable for purification, detection, and immobilization of biotinylated molecules:

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

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1 – 15 of 526 results
1

IBA Lifesciences Biotin
GREENER_CHOICE

EDGE
2

IBA Lifesciences Desthiobiotin
GREENER_CHOICE

Desthiobiotin powder for preparation of Strep-Tactin™ elution buffer

EDGE
Product Type Desthiobiotin
For Use With (Application) For Elution of Strep-Tag II Proteins From Strep-Tactin
3

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Spin Column Kit

Strep-Tactin™XT 4Flow™ high capacity Spin Column Kit for rapid purification of Strep-tag™II and Twin-Strep-tag™ fusion proteins.

EDGE
4

IBA Lifesciences pASK-IBA E. coli vector
GREENER_CHOICE

pASK-IBA E. coli expression vectors encoding a Strep-tag™II and chloramphenicol resistance

EDGE
Promoter Tet
Antibiotic Resistance Bacterial Chloramphenicol
Format Suspension
Replication Origin F1 and ColE1 origin
Storage Buffer TE buffer
Cloning Method Direct Cloning
Concentration 250 ng/μL
Size Range approx. 3 kb
Expression System E. coli
Protein Tag Strep-tag II
5

IBA Lifesciences MagStrep Strep-Tactin™ XT Beads
GREENER_CHOICE

MagStrep Strep-Tactin™ XT beads are magnetic beads (5% suspension) to purify Strep-tag™ II or Twin-Strep-tag™ fusion proteins.

Particle Size 20 to 40 μm
Type Magnetic Agarose Bead
Target Twin-Strep-tag or Strep-tag II
Form Resin Slurry
Formulation 5% (v/v) Suspension in PBS with 0.1% (w/v) Sodium Azide
Support Type 6% Agarose, Crosslinked
6

IBA Lifesciences 10x Buffer BXT

IBA Lifesciences GmbH 10x Buffer BXT; Strep-Tactin™XT Elution B. is a ready-to-use buffer for elution of Strep-tagged proteins from Strep-Tactin™ XT.

7

IBA Lifesciences BioLock
GREENER_CHOICE

BioLock is a Biotin blocking solution for lysates and culture supernatants

Type Blocking Solution
8

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity 50% suspension

Strep-Tactin™ 4Flow™ high capacity 50% Suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Particle Size 50 to 150 μm
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Type Bulk Resin
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Format Bottle
Description 4% agarose, crosslinked
Final Product Type Affinity chromatography equipment
Protein Form Tetrameric protein
Form Resin Slurry
Binding Capacity 20 mg/mL resin
Column Type Suspension
Concentration 50% suspension
9

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Resin
BIOPHARMA

Strep-Tactin™XT 4Flow™ high capacity resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

10

IBA Lifesciences 10x Buffer W - Wash Buffer

Strep-Tactin™ and Strep-Tactin™XT wash buffer

11

IBA Lifesciences Strep-Tactin™XT 4Flow™ Resin
BIOPHARMA

Strep-Tactin™XT 4Flow™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

12

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity FPLC column

Strep-Tactin™ 4Flow™ high capacity FPLC column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Particle Size 50 to 150 μm
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Type Column
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Description 4% agarose, crosslinked
Final Product Type Affinity chromatography equipment
Protein Form Tetrameric protein
Form Resin Slurry
Binding Capacity 20 mg/mL resin
Quantity 5 mL
13

IBA Lifesciences MagStrep 'Type3' Strep-Tactin Beads 5% Suspension

MagStrep 'type3' Strep-Tactin beads 5% suspension are magnetic beads to purify biotinylated proteins.

14

IBA Lifesciences Biotin stock solution
GREENER_CHOICE

For dissociation of Twin-Strep-tag™ from Strep-Tactin™ suitable for cells

15

IBA Lifesciences Strep-Tactin™ Conjugate

Strep-Tactin™ conjugates with AP or HRP for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins

Cell Selection and Expansion

Filename
ApplicationNote-Central-memory-T-cells-isolated-and-stained-with-CD8α-and-CD62L-Fab-Streptamer-reagents.pdf
Size
662 KB
Format
application/pdf

Application Note Combining MHC-I and CD8+ Fab Streptamer reagents

Filename
ApplicationNote-Combining-MHC-I-and-CD8+-Fab-Streptamer-reagents.pdf
Size
803 KB
Format
application/pdf
Filename
ApplicationNote-Regulatory-T-cells-isolated-and-stained-with-CD4-CD25-and-CD45RA-Fab-Streptamer-reagents.pdf
Size
737 KB
Format
application/pdf
Filename
ApplicationNote-CD3-T-Lymphocytes-isolated-and-stained-with-Streptamer-reagents.pdf
Size
200 KB
Format
application/pdf
Filename
Application_Note_Negative_cell_selection_using_affinity_chromatography.pdf
Size
1 MB
Format
application/pdf
Filename
Application_Note_Depletion_of_cell_populations_from_different_samples.pdf
Size
1 MB
Format
application/pdf
Filename
White_Paper_Understanding_The_World_of_MHC_I_Multimers.pdf
Size
5 MB
Format
application/pdf

Protein Production and Assay

ApplicationNote-Strep-Tactin-XT-vs-Strep-Tactin-High-Capacity

Filename
ApplicationNote-Strep-Tactin-XT-vs-Strep-Tactin-high-capacity.pdf
Size
193 KB
Format
application/pdf
Filename
ApplicationNote-Strep-Tactin-high-capacity.pdf
Size
100 KB
Format
application/pdf
Filename
ApplicationNote-Strep-Tactin-XT-Assay.pdf
Size
261 KB
Format
application/pdf

IBA-Application Note_STvsXT.indd

Filename
ApplicationNote-Strep-Tactin-vs-Strep-Tactin-XT.pdf
Size
917 KB
Format
application/pdf
Filename
ApplicationNote-Twin-Strep-tag-vs-His-tag.pdf
Size
675 KB
Format
application/pdf

BLI sSPR8 - Kopie.indd

Filename
Application_note_BLISPR_with_Strep-TactinXT.pdf
Size
1 MB
Format
application/pdf
Filename
White_paper_Comparison_protein_purification_systems.pdf
Size
1 MB
Format
application/pdf

20191111_ResearchGate_Expi.indd

Filename
White_paper_Transient_expression_in_mammalian_cells.pdf
Size
1 MB
Format
application/pdf

Exosomes

Application_note_exosomes_MSC.indd

Filename
Application_note_exosome_isolation_from_MSC.pdf
Size
1 MB
Format
application/pdf

Application_note_exosomes_Jurkat_Serum.indd

Filename
Application_note_exosome_isolation_from_Jurkat_Serum.pdf
Size
782 KB
Format
application/pdf