Western Blotting Reagents, Membranes, Supplies
Molecular Probes™ Qdot™ 605 Biotin Conjugate Kit
Qdot™ 605 Biotin Conjugate Kit
Merck Immobilon™-FL PVDF Membranes
Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45 μm pores and have lower background fluorescence than other membranes. Available in 10 and 20 cm squares and a 3.75 m roll.
Cytiva Amersham™ Hyperfilm™ ECL
Use with all blue and green light chemiluminescent systems. Cytiva Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.
Merck Immobilon™ Western Chemiluminescent HRP Substrate (ECL)
Provides high sensitivity over a broad detection range at an economical price
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
Thermo Scientific™ Pierce™ Streptavidin
Purified recombinant streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods including western blotting, ELISA, immunohistochemistry, and fluorescence imaging.
Thermo Scientific™ Pierce™ CN/DAB Substrate Kit
Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.
Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, regular size
Used to transfer proteins with the iBlot 2 Gel Transfer Device
Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution
Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.
Invitrogen™ Mini Blot Module
Wet transfer device used exclusively with the Bolt Minigel Tank
Cytiva Whatman™ Grade GB003 Blotting Paper
Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.
Epredia™ Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer (Ready-To-Use)
A one-step polymer system providing increased sensitivity, time savings and detection simplicity.
Invitrogen™ Shrimp Alkaline Phosphatase (SAP)
Treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. Shrimp Alkaline Phosphatase (SAP) is useful in molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes.
Thermo Scientific™ Western Blot Roller
A simple, reusable tool for gently and easily removing trapped air bubbles when assembling transfer stacks in semi-dry or wet-tank transfer cassettes.
Thermo Scientific™ PVDF Transfer Membranes, 0.45 μm
Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for western, Southern, and northern blotting methods.
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis
Invitrogen™ CellROX™ Orange Reagent, for oxidative stress detection
CellROX™ Orange Reagent is a novel fluorogenic probe for measuring oxidative stress in live cells. This cell-permeant dye is non-fluorescent while in a reduced state and exhibits bright orange fluorescence upon oxidation by reactive oxygen species (ROS), with absorption/emission maxima of ∼545/565 nm.
Invitrogen™ Mini Gel Tank and Blot Module Set
Provides all of the necessary devices for protein separation and Western blotting using Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels
Thermo Scientific™ Pierce™ Protein-Free Blocking Buffer
Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.
Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 μm, 8.3 x 7.3 cm (for mini gels)
Pre-cut and pre-assembled and ready for protein blotting/transfer
Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate
Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.
Thermo Scientific™ Blocker™ BLOTTO in TBS
Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Invitrogen™ iBlot™ Transfer Stack, nitrocellulose, regular size
iBlot Transfer Stacks are used to transfer proteins.
Cytiva Amersham™ ECL™ Western Blotting Detection Reagents
Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.
Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls
Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Epredia™ UltraVision LP Detection System
Epredia™ Lab Vision UltraVision LP Detection System: HRP Polymer is a sensitive detection system based on biotin-free, polymer technology.
Invitrogen™ iBlot™ 2 Electrode Replacement Kit
For use with the iBlot 2 Gel Transfer Device
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.